The “Emotion Sensing for (E-)Bicycle Safety and Mobility Comfort” approach, in short, “ESSEM”, funded by the mFUND program of the Federal Ministry of Digital Affairs and Transport (BMDV), investigates the subjective safety perception of cyclists in urban traffic. Identifying Moments of Stress in the bicycle network in Ludwigsburg and Osnabrück is done by collecting biophysiological data using sensor technologies and surveys. Besides developing a practical tool for evaluating bicycle infrastructures with emotion-sensing data, bicycle components are designed and assessed within the project.